Detection of Differentially Expressed Genes in Hepatocellular Carcinoma Using DNA Microarray
JGG&2000,&27(12):&&DOI:
&&ISSN:&&CN:&11-5450/R
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Detection of Differentially Expressed Genes in Hepatocellular Carcinoma Using DNA Microarray
LI YQIU Min-YWU Chao-Q CAO Yue-Q TANG R CHEN Q SHI Xue-YHU Zhi-QXIE Yi;MAO Yu-Ming
1:State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, 200433 China
2:United Gene Holdings, Ltd, Shanghai 200092,China
3:Shanghai Changzheng Hospital, the Second Military Medical University,Shanghai, 200003 China
Abstract：
Large scale gene expression profiles berween normal hepatocytes and hepatocellular carcinomas are detected with high throughput microarray. DNA microarrays were made by spotting PCR products of 4 096 human cDNAs onto a specially treated glass slides. The probes were perpared by labelling normal tissue
mRNA and cancer tissue mRNA with Cy3-dUTP and Cy5-dUTP separately through reverse transcription. The arrays were then hybridized againest the cDNA probe mixture and the fluorescent signals were scanned. The obtained data were analyzed from 4 repeated experiments. There are 903 genes of which the average ratios between Cy3 signal and Cy5 signal are greater than 3.0 These genes identified through this approach are potential candidates for hepatocellular carcinoma diagnosis and therapy.
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/topic/zui-yi-xiao-hong-chen-zhuan-lan-xie-dian-bu-yi-yang/
/topic/su-du-he-si-ji-huo-dong-jie-shu/
/topic/jian-dao-shou-ji/
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/topic/li-qi-IO/
/topic/mi-tu-sui-yi/
/topic/yao-tiao-shu-nv/
/topic/gui-hua-lian-pian/
/topic/zhua-zhu-shi-jian-de-xiao-jiao-ya/
/topic/mi-shi-zai-bei-jing/
/topic/xue-sheng-shi-dai-de-jian-ying/
/topic/si-shi-jie/
/topic/nao-ke-xue-jiao-du-liao-liao-yi-yu-zheng/
/topic/cai-hong-qi-xia/
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/topic/jia-zheng-fu-wu-yu-biao-zhun/
/topic/xin-li-xue-xi-bi-ji-he-an-li/
/topic/chuang-ke-zhi-xuan/
/topic/xia-ma-wen-qian-cheng/
/topic/gong-zuo-shi-wo-kuai-le/
/topic/xi-you-nao-dong/
/topic/IT-shuo/
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/topic/lian-he-ban-gong/
/topic/ru-he-li-cai/
/topic/hu-bu-yu/
/topic/ming-ming-jiu/
/topic/cheng-shi-feng-jing/
/topic/ke-jia-wen-hua/
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/topic/yin-le-za-ji/
/topic/luo-hou-xiao-sheng-de-du-shu-bi-ji/
/topic/jiang-ge-gu-shi-gei-ni-ting/
/topic/ke-ji-pai/
/topic/ran-er-wo-jing-ran-xiang-dang-yi-zhi-you-qu-de-chan-pin-gou/
/topic/fei-wu-zhi-wen-hua-yi-chan/
/topic/wo-men-de-sheng-huo-suo-shi/
/topic/shi-fou/
/topic/lin-quan/
/topic/feng-yu/
/topic/mei-tian-wei-ni-du-shu/
/topic/chuan-bo-mei-jie-chuan-bo-chuan-bo-mei-jie-ren-yu-she-hui/
/topic/xi-ju-de-biao-yu-li/
/topic/yu-feng-xian-bo-yi-de-bao-xian-xue/
/topic/xiang-you-wang-qu-shi-bao-dao/
/topic/she-hui-ze-ren/
/topic/xian-dai-yan-ke-yi-sheng-zhi-shi-kuo-zhan-bao/
/topic/zhi-shen-yu-ruo-you-ruo-wu-zhi-jian/
/topic/ci-yuan/
/topic/cang-yu/
/topic/I-M-A-DREAM-TELLER/
/topic/PLANTS-ARE-FRIENDS/
/topic/fan-du-zi-zuo/
/topic/cai-hong-xiao-ma-ta-tiao-yue-zai-nao-dong-zhi-jian/
/topic/ce-shi-yong-zhu-ti/
/topic/ji-jian-zhu-yi-chi-huo/}