关注今日：1 | 主题：118419
微信扫一扫
用NEGA5.O在mega中怎么分别计算每对序列之间的dN和dS值呀？急求解
页码直达：
这个帖子发布于5年零274天前，其中的信息可能已发生改变或有所发展。
在mega中怎么分别计算每对序列之间的dN和dS值呀？急求解还有生物统计学检验公式Z=(Ka-Ks)/[V│Ka-Ks│]1/2
这里的V所说的方差是怎么算的呀？
不知道邀请谁？试试他们
微信扫一扫
广告宣传推广
政治敏感、违法虚假信息
恶意灌水、重复发帖
违规侵权、站友争执
附件异常、链接失效
关于丁香园Access denied | romhustler.net used Cloudflare to restrict access
Please enable cookies.
What happened?
The owner of this website (romhustler.net) has banned your access based on your browser's signature (b37868-ua98).The GATA1 mutation in an adult patient with acute megakaryoblastic leukemia not accompanying Down syndrome | Blood Journal
The GATA1 mutation in an adult patient with acute megakaryoblastic leukemia not accompanying Down syndrome
Hideo Harigae, Gang Xu, Tomohiro Sugawara, Izumi Ishikawa, Tsutomu Toki and Etsuro Ito
Blood 103:; doi: https://doi.org/10.1182/blood-6
Hideo HarigaeGang XuTomohiro SugawaraIzumi IshikawaTsutomu TokiEtsuro Ito
Mutations of the GATA1 gene, which is located on chromosome X, have been found in almost all cases of transient myeloproliferative disorder (TMD) and acute megakaryoblastic leukemia (AMKL) accompanying Down syndrome (DS).- No GATA1 mutations have been detected in patients with AMKL who did not have DS, except in AMKL with acquired trisomy 21.,, However, because only 10 cases with AMKL in non-DS have been analyzed for mutations of the GATA1 gene, it remains unknown whether the GATA1 mutation is exclusively involved in the development of AMKL with DS. Here, we report for the first time a GATA1 mutation in AMKL cells from a patient who did not have DS or acquired trisomy 21.
In October 2002, a 48-year-old woman was admitted to Furukawa City Hospital with complaints of shortness of breath and a bleeding tendency. Peripheral blood analysis showed severe anemia, thrombocytopenia, and mild leukocytosis with the appearance of immature cells: red blood cell count, 87 × 1012/L; hemoglobin, 27 g/L (2.7 g/dL); platelet count, 6 × 109/L; and white blood cell count, 10.8 × 109/L (neutrophils, 29%; basophils, 1%; lymphocytes, 46%; blasts, 16%; metamyelocytes, 2%; myelocytes, 3%; and promyelocytes, 3%). Pathologic examination of her bone marrow showed a mixture of dominantly proliferated small lymphocytic cells, which were positive for leukocyte common antigen (LCA) and CD79α, and scattered blastic cells, which were negative for myeloperoxidase. Chromosomal analysis revealed 47XX, add(17)(p11), +add(17), add(20)(q13). The patient was diagnosed with acute lymphoblastic leukemia, chemotherapy was given, and complete remission was achieved. In February 2003, the disease relapsed and became refractory to chemotherapy. The morphology of leukemic cells at the relapse resembled that of small megakaryocytes, and they were negative for myeloperoxidase and positive for acid phosphatase. Immunohistochemical examination revealed that the leukemic cells were positive for CD33, factor VIII, and CD41a, and dull-positive for CD4 and CD45. In addition, flow cytometric analysis using an anti–GATA-1 antibody revealed that the leukemic cells were positive for GATA-1. The same chromosomal anomaly as that detected at onset was detected by chromosomal analysis. Based on these results, she was finally diagnosed as having AMKL.
We analyzed the GATA1 mutation in her bone marrow samples. Written informed consent was obtained from the patient. Genomic DNA was extracted and cDNA was constructed, and then polymerase chain reaction was performed and the products were sequenced directly, as described previously. An insertion of 20 nucleotides corresponding to a sequence in exon 2 of the GATA1 gene was detected, resulting in the introduction of a premature stop codon in the gene sequence encoding the N-terminal activation domain ().
GATA1 mutation in an adult patient with AMKL not accompanying DS. Direct sequence analysis of cDNA and genomic DNA from AMKL blast cells of the adult patient who did not have accompanying DS showed that 20 nucleotides of the duplicated sequence were inserted in exon 2 of the GATA1 gene. The mutation resulted in the introduction of a premature stop codon in the gene sequence encoding the N-terminal activation domain. The predicted protein from a downstream initiation site, GATA-1s, lacks the transactivation domain.
Our results suggest that GATA1 mutations play an important role in the development of AMKL in non-DS individuals. Our discovery warrants further investigation in a larger series of non-DS patients with AMKL.
Footnotes Correspondence: Etsuro Ito, Department of Pediatrics, Hirosaki University School of Medicine, Hirosaki, Aomori, 036-8563 J e-mail: eturou{at}cc.hirosaki-u.ac.jp. References
Wechsler J, Greene M, McDevitt MA, et al. Acquired mutations in GATA1 in the megakaryoblastic leukemia of Down syndrome. Nat Genet. ;32: 148-152.
Mundschau G, Gurbuxani S, Gamis AS, et al. Mutagenesis of GATA1 is an initiating event in Down syndrome leukemogenesis. Blood. ;101: 4298-4300.
Hitzler JK, Cheung J, Li Y, et al. GATA1 mutations in transient leukemia and acute megakaryoblastic leukemia of Down syndrome. Blood. ;101: 4301-4304.
Rainis L, Bercovich D, Strehl S, et al. Mutations in exon 2 of GATA1 are early events in megakaryocytic malignancies associated with trisomy 21. Blood. ;102: 981-986.
Groet J, McElwaine S, Spinelli M, et al. Acquired mutations in GATA1 in neonates with Down's syndrome with transient myeloid disorder. Lancet. ;361: 1617-1620.
Xu G, Nagano M, Kanezaki R, et al. Frequent mutations in the GATA-1 gene in the transient myeloproliferative disorder of Down's syndrome. Blood. ;102: 2960-2968.
Miura T, Yokoyama H, Minegishi N, Sasaki T, Kaku M, Harigae H. Flow cytometry of GATA transcription factors. Cytometry. ;56B: 1-7.
Volume: 103
DOI: https://doi.org/10.1182/blood-6
Thank you for sharing this Blood Journal article.NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.
Your Email *
Your Name *
Enter multiple addresses on separate lines or separate them with commas.
You are going to email the following
Message Subject
(Your Name) has forwarded a page to you from Blood Journal
Message Body
(Your Name) thought you would be interested in this article in Blood Journal.
Your Personal Message
User Name *
Password *
Sign In to Email Alerts with your Email Address
Hideo Harigae, Gang Xu, Tomohiro Sugawara, Izumi Ishikawa, Tsutomu Toki, Etsuro Ito
Blood 103 (8) ; DOI: 10.1182/blood-6
Citation Manager Formats
Harigae, H., Xu, G., Sugawara, T., Ishikawa, I., Toki, T., & Ito, E.
The GATA1 mutation in an adult patient with acute megakaryoblastic leukemia not accompanying Down syndrome.
Accessed July 27, 2018.
Hideo Harigae, Gang Xu, Tomohiro Sugawara, Izumi Ishikawa, Tsutomu Toki, Etsuro Ito
Blood 103 (8) ; DOI: 10.1182/blood-6
Advertisement
Related Articles美嘉音响-数字音频处理器-DP448-DP480-DP48-DSP48-DS48
厂家报价：面议
产品状态：(在产)
产品介绍：
DS48DP48DP480DP448数字音箱处理器数字音频处理器数字分频器音箱处理器音频处理器
产品名称：DP448-DP480-DP48-DSP48-DS48
产品品牌：
产品类别：
数字音频处理器
厂家联系方式：
地址：广州市番禺区钟村镇人民路波兰社大街25号
厂商介绍：
广州美嘉专业音响器材厂（美嘉MEGA）主要生产的产品有数字音频处理器、数字音箱处理器、数字处理器、音箱相位检测仪、音响相位测试仪、话筒相位测试仪、DSP数字效果器、前级效果器、数字功放板、音箱控制器、...
DS48DP48DP480DP448数字音箱处理器数字音箱处理器
产品名称：DP448-DP480-DP48-DSP48-DS48
主要特点：
※精选双精度的DSP处理器和32bit的内部数据通道，具有特别宽阔的动态范围和高保真的音色。
※采样率高，损耗低，动态范围可达＞105dB。
※方便灵活多分频模式，DP48=4路输入8路输出处理，机内已配置10种工作模式，4 x 2-way C 2 x 3-way, and 2 A2 x 3-way + 2 x 4-way C 1 x 5-way, and 3 A LCRS 4 x4 P Muted, Flat Startup并带有限幅器，在各种配置模式中，所有参数均可调整。
※每个参数段的增益调整范围为+6dB至-40dB，中心频率范围为20HZ-20KHZ，Qs范围为0.5到128，每个输入通道都包括输入增益控制，每一个通道都包括独立的分频限制器，输出均带有5段参量均衡，（PEQ）带宽范围为1/36到4倍频程（Oct），参数可进行大范围调整，用于优化系统的频率响应。每次开机各通道输出音量渐出，更稳定的保护了音乐信号对音箱系统的瞬间冲击。
※每个输出通道有独立的静音按键。每个输出通道都有一个独立的限幅器，其启动时间、释放时间和门槛值等参数均可在大范围内调整；每个输入与输出端有7个LED电平显示，电平表显示是相对于门槛值的动态余量，电平表的时间常数会自动跟踪限幅器的时间常数，使指示更加精确。
※可变的高通虑波器和低通虑波器的斜率可设置为：6dB、12dB、18dB、24dB、36dB或48dB每倍频程，并可选择其响应为：巴特沃斯（Butterworth）、林克维茨-瑞莱（Linkwitz-Riley）、贝塞儿（Bessel）及12dB的多种可变值斜率选择；高通及低通虑波器的参数可以独立调整，能够实现不对称的分频功能。
※任意通道之间参数设置可以自由复制，对系统各通道参数进行调整时更、方便。输出增益及相位控制，参数加锁，避免误操作. 每个通道包括输入增益控制，独立的分频限制器
※三个电压传感型360&旋转编码器，用于控制所有参数调整，操作符合一般人的操作习惯。在调整时，（LCD）上会同步显示出各项参数值。
※每路输出通道延时独立可调整，最大延时为7mS，最小调整步距为0.021mS。
※所有设置的状态可保存在记忆中，并且有关机自动保存设置功能，30个用户自定义存储模式，USB和RS232串口联机接口，可由PC电脑操作本机，在PC控制软件友好直观的操作界面上做所有的预设、调试操作更加方便简单，稳定性强，性价比高，更即人性化，以及多级完善的安全锁功能。
※20X2蓝色背光源白色字符LCD显示屏，显示更清楚直观，12X7段输入输出精确数字电平表；外形尺寸：482mm*200mm*44mm。
※电源采用供电，宽频电压输入，从AC90-AC250/50-60Hz，速度更快，自动调整，适用范围更广，稳定性更高，适合全球电压使用。
※配有PC操作软件，可以通过USB或RS232端口实现远程实时控制，可通过网络集线器来实现多达256台的系统集连，可选择设备ID端口自由操控任意一台设备。
您还能输入500字后使用快捷导航没有帐号？
请完成以下验证码
查看: 3329|回复: 4
请教AVRMega128访问DS3231SN数据不正确原因
在线时间4 小时
TA的帖子TA的资源
一粒金砂（中级）, 积分 20, 距离下一级还需 180 积分
一粒金砂（中级）, 积分 20, 距离下一级还需 180 积分
最近搞个板子，使用Mega128访问DS3231SN，可是数据读取始终为0，不知何故，请大家帮忙看一下代码，看看哪有问题，谢谢。 上拉电阻为10K。
#ifndef&_ds3231_h#define&_ds3231_h //==================================================================== #define&RST_On&CLR_BIT(PORTB,PB6) #define&RST_Off&SET_BIT(PORTB,PB6) #define&GET_SDA&GET_BIT(PINA,PA3) #define&SDA_High&SET_BIT(PORTA,PA3) #define&SDA_Low&CLR_BIT(PORTA,PA3) #define&SDA_In&CLR_BIT(DDRA,PA3) #define&SDA_Out&SET_BIT(DDRA,PA3) #define&SCL_High&SET_BIT(PORTA,PA2) #define&SCL_Low&CLR_BIT(PORTA,PA2) #define&SCL_Out&SET_BIT(DDRA,PA2) struct&DS3231_DAT { &&&&uchar& uchar& uchar& uchar& uchar& uchar& &&&&float& }*ds3231; void&I2C_Start(void) { &&&&SDA_O SDA_H SCL_H delay_nus(5); SDA_L delay_nus(5); SCL_L delay_nus(5); } void&I2C_Stop(void) { &&&&SDA_O SDA_L SCL_H delay_nus(5); SDA_H delay_nus(5); } uchar&I2C_Write(uchar&dat) {&&& &&&&uchar&i, SDA_O for(i=0;i&8;i++) { &&&&SCL_L delay_nus(5); &&&&if(dat&0x80) { &&&&SDA_H LED_ON(); } else { &&&&SDA_L LED_OFF(); } delay_nus(5); SCL_H &&&&&&&&delay_nus(5); dat&&=1; } //read&the&ack&bit SDA_In; SDA_H SCL_L delay_nus(5); SCL_H delay_nus(5); ret=GET_SDA; SCL_L delay_nus(5); return& } void&I2C_Write_byte(uchar&add,uchar&data) { &&&&//I2C_Start(); I2C_Write(0xd0);//Write&mode I2C_Write(add); I2C_Write(data); //I2C_Stop(); } uchar&I2C_Read(uchar&ackBit)//ackBit:&low&for&ACK,&high&for&None&ACK { &&&&uchar&i,dat=0x00; &&&&SDA_In; &&&&SCL_L delay_nus(5); for(i=0;i&8;i++) { &&&&SCL_H &&&&&&&&delay_nus(5); dat|=GET_SDA; dat&&=1; SCL_L &&&&&&&&delay_nus(5); } //Send&ACK &&&&SDA_O if(ackBit==0) { &&&&SDA_L } else { &&&&SDA_H } SCL_H delay_nus(5); SCL_L delay_nus(5); SDA_In; return& } uchar&I2C_Read_byte(uchar&addr,uchar&ackBit)//ackBit:&low&for&ACK,&high&for&None&ACK { &&&&uchar& //I2C_Start(); I2C_Write(0xd1);//read&mode I2C_Write(addr); dat=I2C_Read(ackBit); //I2C_Stop(); return& } void&Ds3231_Set_Time(uchar&year,uchar&month,uchar&day,uchar&hour,uchar&minute,uchar&second) { &&&&I2C_Start(); I2C_Write_byte(0x00,num2BCD(second,10)); I2C_Write_byte(0x01,num2BCD(minute,10)); I2C_Write_byte(0x02,num2BCD(hour,10)); I2C_Write_byte(0x04,num2BCD(day,10)); I2C_Write_byte(0x05,num2BCD(month,10)); &&&&I2C_Write_byte(0x06,num2BCD(year,10)); I2C_Stop(); } void&DS3231_init(void) { &&&&OE_On; SDA_O SCL_O SCL_H SDA_H I2C_Start(); I2C_Write_byte(0x0e,0x00);//enable&oscillator I2C_Write_byte(0x0f,0x00); I2C_Stop(); //&12:11:10 Ds3231_Set_Time(11,8,10,12,11,10); } void&getDs3231Temp(void) { &&&&uint&hValue,lV uchar& float& I2C_Start(); //check&if&bus&is&busy while(I2C_Read_byte(0x0f,0)&0x04); //write&convert&cmd I2C_Write_byte(0x0E,0x20); //wait&until&busy=0&and&conv=0& while((I2C_Read_byte(0x0f,0)&0x04)||(I2C_Read_byte(0x0E,0)&0x20)); //read&high&byte hValue=I2C_Read_byte(0x11,0); //get&sign sign=hValue&0x80; //remove&sign hValue=hValue&0 //read&low&byte lValue=I2C_Read_byte(0x12,1)&&6; //combine&the&temp&code I2C_Stop(); temp=hValue&&2|lV& //add&sign if(sign&0) { &&&&temp*=-1; } ds3231-&temperature=&0.03125&*//convert&to&degree } void&getFullTimeNow(void) { &&&&I2C_Start(); ds3231-&year=I2C_Read_byte(0x06,0); ds3231-&month=I2C_Read_byte(0x05,0); ds3231-&day=I2C_Read_byte(0x04,0); ds3231-&hour=I2C_Read_byte(0x02,0); ds3231-&minute=I2C_Read_byte(0x01,0); ds3231-&second=I2C_Read_byte(0x00,1); //getDs3231Temp(); I2C_Stop(); } uchar&getYear(uchar&ackBit)//ackBit:&low&for&ACK,&high&for&None&ACK) { &&&&return&I2C_Read_byte(0x06,ackBit); } uchar&getMonth(uchar&ackBit) { &&&&return&I2C_Read_byte(0x05,ackBit); } uchar&getDay(uchar&ackBit) { &&&&return&I2C_Read_byte(0x04,ackBit); } uchar&getHour(uchar&ackBit) { &&&&return&I2C_Read_byte(0x02,ackBit);; } uchar&getMinute(uchar&ackBit) { &&&&return&I2C_Read_byte(0x01,ackBit); } uchar&getSecond(uchar&ackBit) { &&&&return&I2C_Read_byte(0x00,ackBit); } void&resetTime(void) { &&&&RST_On; delay_nms(250); RST_O&&& } //==================================================================== #endif
在线时间4 小时
TA的帖子TA的资源
一粒金砂（中级）, 积分 20, 距离下一级还需 180 积分
一粒金砂（中级）, 积分 20, 距离下一级还需 180 积分
下面是主函数
void main(void)
&&& //init ds3231
&&& Ds3231_init();
&&& Ds3231_Set_Time(11,8,6,9,10,20);&&& Uart0_SendStr("\nSystem time set OK.\n");
&&& while(1)
&&&&&&&& I2C_Start();&&&&&&&& Uart0_SendStr("\nControl Register=");&&&&&&&& Uart0_SendStr(num2Str2(I2C_Read_byte(0x0e,0)));&&&&&&&& Uart0_SendStr("\nStatus Register=");&&&&&&&& Uart0_SendStr(num2Str2(I2C_Read_byte(0x0f,0)));
&&& &&&& Uart0_SendStr("\nYear=");&&&&&&&& Uart0_SendStr(num2Str2(I2C_Read_byte(0x06,0)));&&&&&&&&&Uart0_SendStr(",Month=");&& Uart0_SendStr(num2Str2(I2C_Read_byte(0x05,0)));&&&&&&&& Uart0_SendStr(",day=");&&&&&&&& Uart0_SendStr(num2Str2(I2C_Read_byte(0x04,0)));&&&&&&&& Uart0_SendStr(",Hour=");&&&&&&&& Uart0_SendStr(num2Str2(I2C_Read_byte(0x02,0)));&&&&&&&& Uart0_SendStr(",Minute=");&&&&&&&& Uart0_SendStr(num2Str2(I2C_Read_byte(0x01,0)));&&&&&&&& Uart0_SendStr(",Second=");&&&&&&&& Uart0_SendStr(num2Str2(I2C_Read_byte(0x00,1)));&&&&&&&& Uart0_Send('\n');&&&
&&&&&&&& I2C_Stop();
[ 本帖最后由 doleph 于
11:13 编辑 ]
在线时间988 小时
芯币2749枚
E金币128枚
TA的帖子TA的资源
一粒金砂（高级）, 积分 340, 距离下一级还需 160 积分
一粒金砂（高级）, 积分 340, 距离下一级还需 160 积分
不知楼主最后是怎么解决问题的呢& &我现在调STM8+DS3231SN&&也是读不到数据&&很悲催啊，程序是从51上移植过来的，51的已经批量使用&&
我从不担心我努力了不优秀，只担心优秀的人都比我更努力。如果你无法忍受孤独，就不要追逐梦想。每一个优秀的人，都有一段沉默的时光。在那一段时光，你付出了很多努力，忍受孤独和寂寞，不抱怨不诉苦，最后渡过了这
在线时间5 小时
TA的帖子TA的资源
一粒金砂（初级）, 积分 0, 距离下一级还需 5 积分
一粒金砂（初级）, 积分 0, 距离下一级还需 5 积分
if(dat&0x80) 是不是对映SDA口线!
在线时间5 小时
TA的帖子TA的资源
一粒金砂（初级）, 积分 0, 距离下一级还需 5 积分
一粒金砂（初级）, 积分 0, 距离下一级还需 5 积分
3口应是if(dat&0x08).
EEWORLD 官方微信
Powered by}